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Home Protocols Recipes Agarose gel electrophoresis

Agarose gel electrophoresis

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50X TAE
mix 242g Tris base, 57.1ml Glacial Acetic Acid, 100ml EDTA (0.5M, pH=8), H20 to 1 L

10 x TBE (890 mM Tris base, 890 mM boric acid, 20 mM EDTA, pH=8):
add 108 g Tris base (= Trizma), 55 g boric acid to 800 mL distilled H2O
add 40 mL EDTA (0.5 M, pH=8)
stir until completely dissolved and adjust volume to 1 L with distilled H2O
TE-buffer (10 mM Tris, 1 mM EDTA, pH=8):
use 10 mL of Tris-buffer (1 M, pH=8) and 2 mL of EDTA (0.5 M, pH=8)
add to 1 L with H2O and autoclave
Tris-buffer (1 M, pH=8):
121 g Trisbase in 800 mL H2O
adjust pH with HCl (concentrated)* add to 1 L with H2O and autoclave
*the pH is temperature dependent (approx. 0.03 units per 1°C), thus wait until buffer reaches room temperature, before making final pH adjustments