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Home Protocols Recipes In-house sequencing

In-house sequencing

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Plasmid sequencing

User provides template + primer mix in 7μL (100ng template/5kb plasmid DNA and 1.6 pmol primer)
Example:
Your plasmid prep is at 50ng/μL and your sequencing primer at 1 pmol/μL.
Mix 2μL of your template (plasmid size=approx. 5kb) with 1.6μL of sequencing primer and fill up to 7μL.

PCR product sequencing
User provides template + primer mix in 7μL (50ng/1000bpPCR product and 0.4 pmol primer; 50 ng=miniumum)